Blar i forfatter "Berg, Kristel"
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Biochemical characterization of ferric uptake regulator (Fur) from Aliivibrio salmonicida. Mapping the DNA sequence specificity through binding studies and structural modelling
Berg, Kristel; Pedersen, Hege Lynum; Leiros, Ingar (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-09)Iron is an essential nutrient for bacteria, however its propensity to form toxic hydroxyl radicals at high intracellular concentrations, requires its acquisition to be tightly regulated. Ferric uptake regulator (Fur) is a metal-dependent DNA-binding protein that acts as a transcriptional regulator in maintaining iron metabolism in bacteria and is a highly interesting target in the design of new ... -
Characterization of an intertidal zone metagenome oligoribonuclease and the role of the intermolecular disulfide bond for homodimer formation and nuclease activity
Piotrowski, Yvonne; Berg, Kristel; Klebl, David Paul; Leiros, Ingar; Larsen, Atle Noralf (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-08-17)The gene encoding MG Orn has been identified from a metagenomic library created from the intertidal zone in Svalbard and encodes a protein of 184 amino acid residues. The <i>mg orn</i> gene has been cloned, recombinantly expressed in <i>Escherichia coli</i>, and purified to homogeneity. Biochemical characterization of the enzyme showed that it efficiently degrades short RNA oligonucleotide substrates ... -
A functional and structural study of three bacterial nucleic acid-interacting proteins. The story of a Ferric Uptake Regulator, an Oligoribonuclease and an ATP-dependent DNA ligase
Berg, Kristel (Doctoral thesis; Doktorgradsavhandling, 2020-02-07)Nucleic acid-interacting proteins are essential players in cellular processes of all living organisms. In the present study, we explore three psychrophilic-derived proteins involved in gene regulation, RNA degradation and DNA ligation in bacterial cells, with one common feature; they perform their activity by interacting with nucleic acids. The Ferric uptake regulator (Fur) is a global transcription ... -
Temperature adaptation of DNA ligases from psychrophilic organisms
Berg, Kristel; Leiros, Ingar; Williamson, Adele Kim (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-02)DNA ligases operating at low temperatures have potential advantages for use in biotechnological applications. For this reason, we have characterized the temperature optima and thermal stabilities of three minimal Lig E-type ATP-dependent DNA ligase originating from Gram-negative obligate psychrophilic bacteria. The three ligases, denoted Vib-Lig, Psy-Lig, and Par-Lig, show a remarkable range of ...